Immunoregulation role of adipose mesenchymal stem cells in J774.1 cells

doi:10.3969/j.issn.2095-4344.2014.10.009

Abstract

Abstract:

BACKGROUND: Recent studies have found that adipose mesenchymal stem cells can inhibit immune responses of T cells, B cells and dendritic cells. But it is unclear whether adipose mesenchymal stem cells are able to regulate the immune responses of macrophages.
OBJECTIVE: To observe the expression of interleukin-6 and tumor necrosis factor α in J774.1 cells co-cultured with adipose mesenchymal stem cells co-cultured.
METHODS: Adipose mesenchymal stem cells at a density of 2×10⁷ per well were inoculated into the upper chamber of the transwell and 24-well plates. Then, J774.1 cells were suspended with cell culture medium containing 1 mg/L lipopolysaccharide and planted into the lower chamber of the transwell and 24-well plates containing adipose mesenchymal stem cells as co-culture group. Meanwhile, inactive J774.1 cells to lipopolysaccharide-activat J774.1 cells served as negative and positive control groups, respectively. After 48 hours, the J774.1 cells were collected to extract the RNA samples. Then, mRNA expressions of interleukin-6 and tumor necrosis factor α were measured by real-time quantitative PCR.
RESULTS AND CONCLUSION: Compared with the positive control group, direct-contact co-culture of
adipose mesenchymal stem cells and J774.1 cells significantly reduced expressions of interleukin-6 and tumor necrosis factor α in J774.1 cells, but non-contact co-culture only reduced interleukin-6 expression. These findings indicate that adipose mesenchymal stem cells can inhibit the immune response of J774.1 cells activated by lipopolysaccharide.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: adipose tissue, mesenchymal stem cells, macrophages, coculture techniques, immunomodulation, interleukin-6, tumor necrosis factor-alpha

CLC Number:

R394.2

Bai Li, Zhang Wei, Pang Chun-yan, Wei Fei-fei, Wang Yong-fu. Immunoregulation role of adipose mesenchymal stem cells in J774.1 cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(10): 1532-1538.

Figures/Tables 5

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